Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis

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Improving Antigenicity of the Recombinant Hepatitis C Virus Core Protein via Random Mutagenesis

In order to enhance the sensitivity of diagnosis, a recombinant clone containing domain I of HCV core (amino acid residues 1 to 123) was subjected to random mutagenesis. Five mutants with higher sensitivity were obtained by colony screening of 616 mutants using reverse ELISA. Sequence analysis of these mutants revealed alterations focusing on W(84), P(95), P(110), or V(129). The inclusion bodie...

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ژورنال

عنوان ژورنال: Journal of Biomedicine and Biotechnology

سال: 2011

ISSN: 1110-7243,1110-7251

DOI: 10.1155/2011/359042